Описание: Protein therapeutics of increasing complexity continue to play a growing role in pharmaceutical market. As a result, understanding the absorption, distribution, metabolism and excretion (ADME) of proteins has become more important to enable engineering strategies designed to optimize therapeutic properties of candidate molecules. To this end it is important to have robust methods, such as mass spectrometry, to characterize potential instability across a variety of sample types.
Intact mass spectrometry of therapeutic proteins offers the highest possible resolution to assess protein/peptide integrity as well as post-translational modifications. Despite the benefits of intact protein mass spectrometry, analysis from serum remains challenging. Enrichment or purification is required prior to mass spectrometry analysis of therapeutic proteins in serum due to high abundance of endogenous serum proteins. The discussion will describe the qualitative and quantitative characterization of mAb and protein stability both in vitro and in vivo by CE-MS and LC-MS.
Лектор: Mei Han, Senior Scientist, Pharmacokinetics & Drug Metabolism (PKDM), Amgen, South San Francisco
Дата: 10 декември 2019, Първа сесия: 16:00 ч. и втора сесия: 19:00 ч.