Описание: "Lipid samples are very complex. A challenge in the global analysis of lipids is the vast number of subspecies and isomers present in biological samples. The diversity is due to variation in linkages and positions of fatty acyl chain, head group modifications, presence of molecular species as isomers or isobars are among some of the greatest challenges to overcome. Lipid stereoisomers may have unique roles in biological processes. It is therefore important to be able to separate the structural and stereoisomers which poses a challenging analytical problem. Reverse phase UHPLC separates lipids according to the hydrophobicity of the fatty acyl chains. It is very common to find coeluting isomers which are very difficult to separate with reverse phase chromatography alone. SFC has the advantage of doing lipid class-based separation. The separation is orthogonal to UHPLC. Very polar lipids elute towards the end of the chromatogram. The disadvantage is again coeluting lipids belonging to the same class. The best of both the worlds would be to interface UHPLC to SFC. The simplest setup of the Agilent 1290 Infinity 2-D system is time-based heart cutting in the first dimension followed by automated injection into the second dimension. The advantage of this approach for the separation of lipids is that coeluting peaks within a time window can be sent for SFC separation in the second dimension to possibly separate the isomers.
In this webinar, we will show the separation of lipids with UHPLC, SFC and the design and implementation of a simple 2-D system for lipid separation."
Лектор: Sheher Banu Mohsin, PhD, Senior Applications Scientist, Agilent Technologies, Inc.