Описание: Preparative liquid chromatography begins with an analytical separation. First, we need to confirm the presence of the target compound in the sample. Then, we must assess whether the estimated amount of the target compound we can recover from the sample justifies the subsequent purification effort. Preparative chromatography is not based on guesswork but is founded solidly on a set of well-documented rules to be followed for optimum results when scaled from an analytical method. Scouting for appropriate starting conditions, optimizing for speed, yield, and purity are fundamental considerations. The desired sample throughput determines priorities: high yields for a few different samples justify optimization of yield, whereas dealing with large numbers of different samples at the milligram scale demands proper automation. This webinar was intended to demonstrate, with a practical example, scale up from analytical to preparative HPLC with different purification goals utilizing very straightforward scale up formulas. The examples will utilize practical considerations such as focused method development, loading studies and understanding the principles of scale up. The Preparative HPLC Calculator is one of the tools available that was developed to improve the ease with which you can scale up your analytical methods to meet your separation goals. We will utilize a Preparative Calculator tool to quickly determine the conditions for our preparative separation with predictable results that will meet the goals of our projects.
Лектор: Lori Sandford, HPLC Applications Scientist, Agilent Technologies, Inc.
Lorena Lopez Pena, LC Application Scientist, Agilent Technologies, Inc.
Дата: 24 февруари 2021, 20:00 ч.
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