Описание: Mass spectrometry (MS) is emerging as a robust and sensitive technology for the study of protein structure. For intact proteins, mass confirmation and putative post-translational modifications are readily identified using denaturing MS. For studying protein structure or non-covalent complexes, native MS is the preferred approach. Native MS, where protein analysis is performed using a non-volatile salt in aqueous buffer at neutral pH conditions, has recently been used routinely for protein analysis. In a biopharmaceutical environment, native MS helps to characterize mAbs, cysteine-conjugated anti-body drug conjugates or non-covalent complexes. Here we will explore different analytical approaches for native MS, including electrospray ionization (ESI), nanoESI, as well as high throughput methods using standard flow, all coupled to Agilent high resolution Q/TOFs and IM-MS QTOF.
Лектор: Caroline Chu, LC/MS Application Scientist, Agilent Technologies, Inc.
Дата: 22 април 2020, 20:00 ч.