Описание: Modern biologics development relies on the screening of 100s - 1000s of monoclonal antibody (mAb) variants to identify lead development candidates with optimal properties such as affinity, specificity, immunogenicity, and glycosylation. Antibody aggregation can be an unintended side-effect of these optimizations as it is influenced by molecular attributes as well as those of the expression host. It is controlled and monitored as a critical quality attribute. The ability to rapidly assess the propensity of mAb to aggregate is thus a core requirement of modern pharmaceutical development. A high-throughput, fluorescence-based aggregation analysis workflow will be presented. This workflow is based on the modular Agilent Cary Eclipse Microplate Reader and commercially available PEPBOPS dye. The workflow provides semi-quantitative estimates of high-molecular weight aggregation in ~ 5 seconds per sample instead of the ~ 5 minutes typically required by more conventional measurement techniques.
Лектор: Dean Brown, Application Scientist, Molecular Spectroscopy, Agilent Technologies, Inc.
Дата: 2 октомври 2020, 20:00 ч. българско време
Продължителност: 22 минути