Описание: When transitioning from discovery proteomics to targeted protein quantification, the multiple reaction monitoring (MRM)-based LC/MS method plays an essential role. Sample complexity and the low concentration of certain proteins are the major challenges encountered when performing protein analysis using targeted methods. Recent advancements in low-flow HPLC and acquisition rates in QQQ instrumentation is enabling MRM-based analytical methods using stable isotope-labeled standard (SIS) peptides for targeted quantitative proteomics to improve sensitivity, linear range, precision, accuracy, robustness, and most importantly throughput in the data acquisition as well as simplified data processing.
This webinar will demonstrate the ease of use of the Evosep One system and will show how in conjunction with the Agilent 6495C QQQ, quantitative proteomics becomes faster and more robust without losing sensitivity.
Лектор: Wendi Hale, PhD, LC-MS Application Scientist, Agilent Technologies, Inc.