Описание: The analysis of vitamin D metabolites is of interest in clinical research labs due to its role in several metabolic processes and it’s essential role in normal functions of biological systems. This fat-soluble vitamin is routinely analyzed in those labs by monitoring the metabolites 25-hydroxyvitamin D2 and D3 (25-HVD2 and 25-HVD3), but while demand for this analysis may have increased, lab space often has not. We will present a highly sensitive and specific LC/MS analytical method developed for the Agilent Ultivo triple quadrupole (TQ) paired with a 1290 Infinity II UHPLC stack, the smallest footprint LC/TQ system available on the market. A simple gradient is used to separate the metabolites, with all monitored analytes eluting in under 5 minutes. Excellent linearity over three orders of magnitude is demonstrated and good peak shape and reproducibility is demonstrated at all levels providing a robust and reliable quantitation.
Лектор: Andre Szczesniewski, Applications Chemist, Agilent Technologies, Inc.
Продължителност: 42 минути
Дата: 17 ноември 2021, 19:00 ч. българско време
Необходимо е да се регистрирате, за да чуете записа на уебинара.