RapidFire 365

The RapidFire 365 High-throughput MS System provides faster, more efficient analyses of functional biochemical assays and samples in biological matrices. The Agilent RapidFire provides automated high-capacity sample analysis, a more successful approach to HTS of challenging drug targets and a 10x faster throughput for MS-based analyses like in vitro ADME assays. It is an end to end solution that delivers shorter time to results, reduced operational costs and automated high-capacity sample processing. RapidFire Integrator is Agilent’s data acquisition and analysis software for the RapidFire High-throughput MS Systems, providing seamless data integration with MassHunter and LIMS. Agilent RapidFire Analyzer software efficiently processes data acquired on the RapidFire 365 platform coupled to an LC/triple quadrupole MS instrument.

  • Enables direct, enzymatic detection of native analytes, without the use of surrogates, radioactivity, coupled assays, or indirect measurements.
  • Provides fast results, with cycle times as fast as 8 sec/sample for drug discovery applications and 10 sec/sample
  • Saves money through reduced sample, solvent, reagent and waste.
  • Increases analysis capacity with automated 63 plate handling and 60 hours (weekend-long) unattended operation for 20,000+ injections.
  • No offline sample preparation
  • Increases productivity and efficiency through time-saving, automated method development routines and solvent switching for multiple different assays in a single batch.
  • Features in-line automated cartridge switching of up to 12 re-usable SPE cartridges, with 2000+ injections per cartridge.
  • Integrated components include Agilent BenchBot Robot for automated plate handling, three Agilent 1200 Quaternary pumps for software-driven solvent switching of up to four solvents per channel, and Agilent QQQ, TOF or QTOF mass spectrometer, and Agilent Mass Hunter data acquisition and analysis software.
  • Excellent data quality through a novel peak detection algorithm
  • Customizable views and desired calculations
  • Fast reporting and data review using Review-by-Exception
  • Customizable export formats
  • Elegant error handling using self-assessment of sampling errors


Supercharge Your Lab's Productivity_Agilent RapidFire 365 High-Throughput Mass Spectrometry System

Bringing Plate Reader Simplicity to Mass Spectrometry

Brochures, English, 19 Dec 2018, 379.91 KB, PDF

Advances in Metabolomics and Lipidomics

Omics Tour

Brochures, English, 29 Oct 2014, 809.71 KB, PDF


Application Notes

High-Throughput (Sub-2.5 Second) Direct Injection Analysis by Mass Spectrometry

A modified RapidFire high throughput method that bypasses SPE desalting and enables a sub-2.5 second sample rate, making analysis of 35,000 samples a day possible

Application Notes, English, 03 May 2019, 1.00 MB, PDF

Ultrafast Analysis of Z-Drugs in Urine Using the Agilent RapidFire High-Throughput Mass Spectrometry System

RapidFire/MS/MS analysis allows for the accurate and precise measurement of a Z-drug panel in urine in under 15 seconds per sample.

Application Notes, English, 13 Mar 2018, 779.09 KB, PDF

High-Throughput Lead Discovery with Agilent RapidFire/MS Systems: Analysis of LpxC

Application Notes, English, 07 Mar 2018, 192.10 KB, PDF

High-Throughput Lead Discovery with Agilent RapidFire/MS Systems: Analysis of Stearoyl-Coenzyme A Desaturase (SCD)

The RapidFire High-throughput Mass Spectrometry System provides drug discovery researchers with mass spectrometry-based...

Application Notes, English, 07 Mar 2018, 270.10 KB, PDF