6545XT AdvanceBio LC/Q-TOF

The Agilent 6545XT AdvanceBio LC/Q-TOF system is designed to handle multiple workflows in biopharmaceutical characterization. When you need to access the most information at the intact protein level, automatically confirm a sequence through peptide mapping, and confidently understand PTMs, Agilent provides a UHPLC Q-TOF that delivers.

Accurately profile major and minor proteoforms with time-of-flight technology offering new levels of sensitivity and clarity to intact protein mass spectrometry. Easily dig deeper into peptide digests with the Iterative MS/MS mode of operation. The 6545XT AdvanceBio Q-TOF is the analytical cornerstone in a comprehensive set of tools designed for biopharma.

  • Get up and running fast with quick-start methods that include Agilent AdvanceBio LC columns for intact protein  and peptide workflows.
  • Characterize both major and minor intact protein variations with an automated data workflow featuring MassHunter BioConfirm software.
  • Adapt your instrument to your needs with SWARM autotune to ensure optimum performance with no manual adjustment. New large molecule setting provides sub-nanogram detection levels for intact glycosylated monoclonal antibodies.
  • Be sure of your results with mass accuracies within 10 ppm for glycosylated intact proteins.
  • Dig deeper into your digested samples with the powerful new iterative MS/MS feature.
  • Maximize your uptime with a system tested to perform through thousands of protein injections without degradation of performance.
  • Minimize maintenance delays with vent-free cleaning of entrance optics.


Agilent 6545XT AdvanceBio LC/Q-TOF System: Designed for Biopharma

Agilent 6545XT AdvanceBio LC/Q-TOF System: Designed for Biopharma

Brochures, English, 23 Apr 2019, 1.53 MB, PDF


Application Notes

Profiling Glycosylation of Monoclonal Antibodies at Three Levels Using the Agilent 6545XT AdvanceBio LC/Q-TOF

This application note demonstrates three approaches to quantitatively understand the glycosylation present for a given mAb.

Application Notes, English, 23 Apr 2019, 1.20 MB, PDF


Precise Characterization of Intact mAbs by the Agilent 6545XT AdvanceBio LC/Q-TOF

This app note presents a complete high-throughput workflow solution to analyze a variety of mAb products.

Application Notes, English, 19 Nov 2018, 1.31 MB, PDF


Host Cell Protein Analysis Using Agilent AssayMAP Bravo and 6545XT AdvanceBio LC/Q-TOF

This study demonstrates an HCP analysis workflow

Application Notes, English, 02 Jul 2018, 1.66 MB, PDF


In-depth Peptide Mapping with Iterative MS/MS Acquisition on the Agilent 6545XT AdvanceBio LC/Q TOF

This Application Note presents an integrated workflow for peptide mapping of a mAb with Iterative MS/MS from automated sample preparation to data analysis.

Application Notes, English, 02 Nov 2017, 2.19 MB, PDF